| Place | Rider | Team | Time |
|---|---|---|---|
| 1 | Sarah Uhl | Penn State | 58:49 |
| 2 | Lauren Franges | Victory Brewing | 58:50 |
| 3 | Sarah Ulmer | Colavita / Cooking Light | 58:50 |
| 4 | Sarah Caravella | TEAm Lipton | 59:00 |
| 5 | Kelly Benjamin | Cheerwine | 59:01 |
| 6 | Chrissy Ruiter | Victory Brewing | 59:01 |
| 7 | Kacey Manderfield | Wolverine / Redken | 59:01 |
| 8 | Iona Wynter | Colavita / Cooking Light | 59:02 |
| 9 | Sarah Tillotson | Colavita / Cooking Light | 59:02 |
| 10 | Caitlyn McCullough | Terry Precision Cycling | 59:02 |
| 11 | Erica Allar | Team Fuji | 59:02 |
| 12 | Laura Yoisten | Victory Brewing | 59:02 |
| 13 | Sheba Farrin | Hub Racing | 59:03 |
| 14 | Megan Guamier | 59:03 | |
| 15 | Julia Farell | Wheels of Bloor | 59:03 |
| 16 | Tiffany Pezzulo | Team Ivory Homes | 59:03 |
| 17 | Gina Voci | Cheerwine | 59:04 |
| 18 | Danielle Haulman | Hot Tubes | 59:04 |
| 19 | Holli Steelman | Cheerwine | 59:04 |
| 20 | Veronica Martinez | Orbea Argentina | 59:04 |
| 21 | Rebecca Wellons | Gearworks / Spin Arts | 59:04 |
| 22 | Kathleen Billington | Diet Cheerwine | 59:04 |
| 23 | Moriah MacGregor | Dizzy Chicks | 59:05 |
| 24 | Nicole Raspa | Hub Racing | 59:05 |
| 25 | Meshy Holt | Colavita / Cooking Light | 59:05 |
| 26 | Anne Guzman | Terry Precision Cycling | 59:06 |
| 27 | Kerry Traynor | Foristar / Handlebars | 59:06 |
| 28 | Kerry Litka | Terry Precision Cycling | 59:06 |
| 29 | Nicole Bossie | Cheerwine | 59:07 |
| 30 | Tara Ross | Victory Brewing | 59:09 |
